Research models

List of models

Nomenclature: BP/2IcRj-BIOZZI

Type: Mutant inbred mouse
Colour and related genotype: albino mouse - Tyrc/Tyrc MHC: Haplotype H2q
Origin: INSERM, Unit 255 (Curie Institute, Paris, France) - 1994
Breeding: Easy to rear

Fields of research

  • Asthma
  • Antibodies production
  • Experimental allergy

Description of our model

High antibody responder descended from selection done by BIOZZI and COL.
The selection for high antibody titer, following a challenge with sheep erythrocytes started in 1968, from outbred SWISS stock. The strain has been bred in a small population for 67 generations.

In mice, 3 loci that influence asthma traits have been identified: the Asthm1 locus (chromosome 9), the Asthm2 locus (chromosome 11) and a locus on chromosome 17 near Bhr3 (Zhang et al., 1999).

In the BP/2 model, an immunization and subsequent challenge with ovalbumin results in asthma symptoms, similar to the ones seen in human allergic asthma: airway inflammation, eosinophil infiltration and non-specific bronchial responsiveness. The locus associated with this respiratory system response is Asthm2. The strain has a high IgE titre and expresses bronchopulmonary hyperresponsiveness (BHR) after an antigenic challenge. BHR is accompanied by airway sequestration of lymphocytes and eosinophils, and by IgE-bearing granulocytes / basophils. After antigen administration, BP2 mice respond with an anaphylactic shock characterized by an intense serotonin-dependent bronchoconstriction, with probable mast cell activation. Immunised and multi-challenged BP2 mice become hyperresponsive to bronchoconstrictor agents, including serotonin, acetylcholine or methacholine. This hyperreactivity lasts several days and probably has an inflammatory etiology because it is suppressed by dexamethasone. Dex also prevents eosinophilia of the airways and production of broncho-alveolar liquid. The airways of BP/2 mice do not respond to exogenous nor endogenous histamine. Isolated airways of BP2 mice contract upon exposure to antigen, to serotonin and cholinergic agents, which correlates in vivo and in vitro results.

Our added value

  • The « JANVIER LABS Genetic Policy », a specific programme, guarantees the homogeneity of the genetic background, identic to the wild types used as controls.
  • Animals with the SOPF standards.
  • A gentling policy for docile and easy-to-handle animals.
  • Optimal stability conditions of our models during shipments, thanks to our dedicated and internal transport service.
  • A scientific support with a team of Veterinarians and PhD.

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