B6 hCD3EDG Mouse
Antibody discovery
Model characteristics
Strain name:
C57BL/6Smoc-Cd3etm1(CD3E)-Cd3dtm1(hCD3D)-Cd3gtm1(hCD3G)/SmocRj
Type :
Humanized mouse
Origin:
SMOC, 2024
Genotype & associated color
Black mouse
Performance de reproduction :
In vivo
Description of our model and application areas
The replacement of the mouse Cd3e/Cd3d/Cd3g genes with their human orthologs enables the preclinical validation of bispecific and multispecific T-cell engagers.
This model exhibits a similar immune cell distribution to that of the wild-type (WT) counterpart. Validation data confirm the binding of several CD3-targeting compounds, each recognizing distinct epitopes.
Tumor growth inhibition and cytokine release induced by candidate CD3xEpCam bispecific compound.
Homozygous hCD3EDG mice were engrafted with MC38-hEpCAM and received treatment with OKT3, CD3xEpCam or vehicle. Tumor growth inhibition results are depicted in A. Body weight evolution (B) and serum cytokine levels (C) results indicate a cytokine release syndrome-like phenotype in mice receiving OKT3, but not candidate compound.
Research model catalog
〉