Quality controls from cryopreserved sperm or embryos

After cryopreserving a strain, it is necessary to monitor the success of freezing and to judge the feasability of subsequent recovery of the strain.

This is the purpose of our quality controls that will provide you with accurate data to be used to optimise the recovery.

Quality control also has the benefit of supplying essential data for a future and efficient recovery.

For frozen sperm (using the JAX™ Sperm Cryo Kit):

• In Vitro quality control (QC1) allows you to know the viability and the ability of thawed sperm to fertilise oocytes.
• In Vivo quality control (QC2) allows you to know the ability of sperm to produce embryos from in vitro fertilisation, able to develop in utero for a live birth.

For frozen embryos:

• In Vitro quality control (QC1) is done by thawing embryos at the 2-cell stage and culturing them for 24 hours. The QC1 is expressed in % of embryos at 4-8 cell stage.
• In Vivo quality control (QC2) is done by thawing embryos at the 2-cell stage and reimplanting them in pseudopregnant females. The QC2 is expressed in % of pups born.