Rapid colony expansion
Rapid colony expansion means producing in a single step as many animals as wanted carrying the genetic modification of the strain.
This is made possible through the use of in vitro fertilisation using a male from the strain and wild type female eggs from donors.
Since the number of females is not limited, it is possible to obtain, in a mastered quantity, a batch of homogeneous animals as regards age, sex and genotype. The animals obtained will be SOPF ensuring a simultaneous rederivation.
The rapid colony expansion provides animals quickly and directly available for experimentation or to start a colony (the use of female wild types implicates a first generation of heterozygous for the transgene).
The rapid colony expansion is also useful for lines with early mortality of individuals (e.g., neuromuscular disease).