NXG Immunodeficient Mouse
Immunodeficient mouse for immunology and oncology research
Inbred transgenic mouse, GEMM
JANVIER LABS, in 2019
Colour and related genotype:
Description of our model and application areas
The NXG or NOD Xenograft Gamma strain is a model of an inbred strain (NOD fund) with 2 mutations of interest, similar to its genetic equivalents NSG, NcG, NOG, etc. (NOD SCID Gamma).
The Prkdcscid mutation, commonly known as “SCID” for “Severe Combined Immunodeficiency”, blocks the development of T and B cells and induces immune deficiency.
Mice homozygous for this mutation show a complete absence of T and B lymphocytes at the periphery.
The IL2rgTm1 mutation called γc is a KO mutation of the gene coding for the c gamma chain that is common (in particular) to interleukins (IL-2, IL-4, IL-7, IL-9 and IL-15). This gene is necessary for the differentiation and the function of numerous hematopoietic cells with a full impact on the development of Natural Killer cells (NK).
The combination of these two mutations Prkdcscid and IL2rgTm1, in NOD funds, induces a severe immunodeficiency with absence of T, B and NK lymphocyte compartments.
The NXG strain was also tested for the polymorphism of the Sirpα gene. The expression of the Sirpα protein (NOD fund alleles) on the surface of macrophages in the bone marrow allows high affinity binding with the CD47 markers of human hematopoietic cells. This binding induces a “don’t eat me” signal that blocks murine macrophages and prevents phagocytosis of transplanted human cells. This is a key feature of the NOD fund which gives an advantage for human transplantation and xenografting in general.
JANVIER LABS obtained the NXG strain (NOD-Prkdcscid -IL2rgTm1/ Rj) by homologous recombination of the IL2rgTm1 mutation called γc (ES cells of B6N mice).
The congenic NOD SCID γc model was obtained by high speed backcross (N = 5).
The gc B6N model was created at the Center of Immunophenomics (Ciphe, Marseille, France) in 2019, and the NOD SCID backcross was performed by Janvier Labs in 2019.
Animals are bred to maintain both the genetic background and the mutations of interest in their homozygous forms.
The inbreeding mode is used for NXG strain and the phenotype is checked according to the JANVIER LABS GENETIC POLICY®.
Main application and research fields
The growth curve represents an average which reflects the weight variation of the strain measured between 21 and 77 days for males and females.
The hematology parameters are an important reference of the B6yc strain based on analyses performed on males and females aged 7-10 weeks. From an immunological point of view, the B6yc strain is characterized by an absence of NK cells, T cells and B cells and a dysfunction of myeloid cells (dendritic and macrophagic cells).
Flow cytometry analysis, spleen
All lymphoid organs of our models were analysed
This model has been entirely characterized. The immunological and hematological parameters were characterized by Center of Immunophenomics (Ciphe, Marseille, France).
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