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B6RAG2 Immunodeficient Mouse

Immunodeficient mouse for immunology and oncology research

Model characteristics

Type:

Mutant inbred mouse, GEMM

Strain name:

C57BL/6NRj-Rag2tm1Ciphe/Rj


Origin:

Ciphe, Marseille France, in 2019

Colour and related genotype:

Black mouse, a (a/a) non agou􀆟 , MHC : Haplotype H2b

Bredding:


Cryo
In vivo
In vitro

Description of our model and application areas

The B6Rag2 (C57BL/6N-Rag2tm1) mouse is an immunodeficient mouse with a Knock Out (KO) genetic mutation in the Rag2 gene (the 2 recombinase activation gene, Rag2tm1) on a C57BL/6N genetic background.

The Rag2tm1 mutation commonly called Rag2 is a KO mutation of the gene coding for the 2 recombinase enzyme that plays a key role in producing T and B receptors of the cells. This lack blocks the development of T and B cells and leads to an immune deficiency.

Homozygous mice for this mutation appear with a total lack of peripheral T and B lymphocyte cells.

The B6Rag2 mouse has proven to be helpful for studies that include, for instance, transplants of allogeneic or syngeneic tumoral stem cells.

The B6Rag2 strain is also helpful in combination with B6Rag2γc and B6γc mice for studies aiming at understanding the role of T, B and NK cells in host resistance to tumors and infectious agents in particular.

JANVIER LABS obtained the B6Rag2 (C57BL/6N-Rag2tm1) through a homologous recombination (ES cells from B6N mice), developed at the Centre d’Immunophénomique (Ciphe, Marseille, France) in 2019.

Whereas other animal models that carry similar genes generally appear with a B6-129s joint genetic background, the JANVIER LABS B6Rag2 strain is specifically and exclusively expressed on a C57BL/6NRj background. Thus the genetic nature of the strain is perfectly controlled and homogeneous. Animals are bred so as to maintain both the genetic background and the mutations of interest in their homozygous forms.

The B6Rag2 strain is bred in an inbred manner and the phenotype is controlled according to the JANVIER LABS GENETIC POLICY®.

Main application and research fields

Physiology - Physiopathology Preclinical

Growth curve

The growth curve represents an average which reflects the weight variation of the strain measured between 21 and 84 days for males and females.

Haematological parameters

Flow cytometry analysis, spleen

All lymphoid organs of our models were analysed

Phenotypic characterisation

This model has been entirely characterized. The immunological and hematological parameters were characterized by Center of Immunophenomics (Ciphe, Marseille, France).

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